Pharmacokinetics and Immunodynamics of Immune stimulating chemotherapeutic nanoparticles for TB
The purpose of this study is to develop a new treatment for tuberculosis (TB). It is expected that direct participation in this study will be during participants' blood draw.
Participants will be asked to participate in a blood draw that will take about 15-30 minutes.
Study visits will be in downtown Buffalo
If you're interested in this study, please contact the study contact listed below.
Cells that fight infection (marcophage) will be isolated from whole blood of healthy donors. All studies are in vitro laboratory studies looking at the effects of nanoparticles on primary macrophage. Primary macrophage are preferred over the use of macrophage cell-lines as they more accurately recapitulate physiology. Macrophage will be incubated with free drug or nanoparticles (concentration: drug MIC) over several half-life increments of each drug.
Supernatant and cell lysate will be collected. Samples will be analyzed by UPLC-MS for intracellular drug concentration; by ELISA for concentrations of cytokines secreted; by ROS assay for reactive oxygen and reactive nitrogen species generation, and; using a calcium detection kit to determine Ca2+ release.
Keywords: Tuberculosis (TB), Macrophages
Willing to serve as a control in the study
Let us know how the study team can reach you. If you do not hear back within 2 business days, reach out to the study team directly at the
contact information above or email email@example.com and someone will assist you.
Your information will be shared only with the research study team for recruitment purposes and designated CTSI personnel for project quality
assurance and will remain confidential. Your information will be stored indefinitely. Should you no longer want this information to be provided
in the aforementioned ways, please contact firstname.lastname@example.org.